|
KMID : 0390220050160020247
|
|
Journal of Clinical Otolaryngology, Head and Neck Surgery
2005 Volume.16 No. 2 p.247 ~ p.252
|
|
|
Apoptosis of p815 Cells by Lidocaine
|
|
Lee Il-Woo
Kwak Jong-Young
Roh Hwan-Jung
Cho Kyu-Sup
Lee Hyun-Sun
Park Hee-Young
|
|
|
Abstract
|
|
|
|
Background and Objectives : Mast cells play a major role in pathogenesis of allergic rhinitis. Lidocaine iswidely used as a short-acting local anesthetic and an anti-arrhythmic agent. Lidocaine also has anti-inflammatory properties by inhibiting the effect of inflammatory cytokines. Although lidocaine is reported to suppress allergic reactions, there have been no report about lidocaine’s direct inhibitory effect on mast cells. The purposes of this study are to identify whether lidocaine directly induces apoptosis of mast cells in vitro and know the pathway of apoptosis. Materials and Methods : After culture of p815 cells, mouse mastocytoma cells, the cells were treated with 2.5 μM, 5 μM lidocaine for 24, 48 and 72 hours and then LD50 of p815 cells was calculated by MTT β3-[4, 5-dimethylthiazol- 2-yl]-2,5-diphenyl-tetrazolium bromide) assay. For identification of apoptosis of p815 cells, flow cytometric analysis in monclonal antibody to annexin V and cell cycle was done. We also estimated the expression of procaspase-3, procaspase-8, procaspase-9, AIF(apoptosis inducing factors) to know the pathway of apoptosis. Results : The LD50 of p815 cells is 3.70 μM after lidocaine treatment for 24 hours, 1.82 μM for 48 hours and 1.72 μM for 72 hours. Lidocaine induced apoptosis in time and dose dependent manner. Procaspase-3 expression was significantly decreased while procaspase-8, procaspase-9 and AIF were not changed. Conclusion :Our results suggest lidocaine can induce apoptosis in mast cells via caspase-3 dependent pathway. Further studies on the pathway of apoptosis and clinical use will be needed.
|
|
|
KEYWORD
|
|
|
Mast cell, Apoptosis, Lidocaine, Allergic rhinitis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|